Modulation of hydroquinone-induced hepatocyte cytotoxicity
| Addition | % Cytotoxicity at time (min) | |||
|---|---|---|---|---|
| 30 | 60 | 120 | 180 | |
| None | 23 ± 2 | 22 ± 2 | 24 ± 3 | 26 ± 2 |
| HQ (200 μM) | 22 ± 2 | 25 ± 3 | 29 ± 3 | 33 ± 2 |
| " + GSH depleted hepatocytes | 45 ± 3 | 46 ± 2 | 48 ± 4 | 62 ± 5 |
| " + dicumarol (25 μM) | 39 ± 4 | 43 ± 4 | 53 ± 5 | 69 ± 7 |
| HQ (2 mM) | 32 ± 2 | 41 ± 4 | 60 ± 43-a | 80 ± 5 |
| " +catalase (100 units/ml) | 20 ± 2 | 23 ± 2 | 40 ± 53-b | 77 ± 3 |
| " + SOD (100 units/ml) | 100 | 100 | 1003-b | 100 |
| " + isoniazid (10 mM) | 25 ± 2 | 34 ± 2 | 36 ± 33-b | 38 ± 2 |
| " +ethylenediamine (2 mM) | 27 ± 3 | 49 ± 4 | 57 ± 3 | 85 ± 3 |
| " + dicumarol (25 μM) | 48 ± 3 | 65 ± 2 | 1003-b | 100 |
| " + deferoxamine (1 mM) | 25 ± 2 | 28 ± 2 | 40 ± 33-b | 60 ± 4 |
| " +pyrogallol (100 μM) | 25 ± 3 | 32 ± 2 | 44 ± 2 | 51 ± 2 |
| " + sorbitol (10 mM) | 28 ± 3 | 33 ± 4 | 36 ± 4 | 47 ± 3 |
Chemicals were incubated with hepatocytes under similar condition to that of Table 2 except that hepatocytes (106 cells/ml) were incubated in Krebs-Henseleit buffer, pH 7.4, at 37 °C under 1% O2/94% N2/5% CO2. Values are expressed as the means of three separate experiments ± S.D. Modulators were not cytotoxic by themselves (data not shown).