Additions | Oxygen Consumed (auto-oxidation) |
---|---|
nmol · min−1 · ml−1 | |
None | 0 |
Hydroquinone (2 mM) | 42 ± 54-a |
" + GSH (1 mM) | 20 ± 34-b |
" + SOD (150 units/ml) | 61 ± 7 |
" + catalase (280 units/ml) | 17 ± 34-b |
" + deferoxamine (1 mM) | 32 ± 64-b |
Autooxidation of HQ was measured from O2 uptake using a Clark O2 electrode. HQ was added to 3 ml of Krebs-Henseleit buffer, containing HEPES (12.5 mM, at 37 °C and pH 7.65). The modulating agents were added approximately 1 min after the addition of HQ.