Sequences of oligonucleotides comprising CYP3A and PXR TaqMan assays
| Assay | Forward Primer (5′-3′) | Reverse Primer (5′-3′) | TaqMan Probe (5′-3′) | Location (exons) |
|---|---|---|---|---|
| CYP3A4-A | TCA GCC TGG TGC TCC TCT ATC TAT | AAG CCC TTA TGG TAG GAC AAA ATA TTT | TCC AGG GCC CAC ACC TCT GCC T | 1–3 |
| CYP3A4-B | TGT CCT ACC ATA AGG GCT TTT GTA T | TTC ACT AGC ACT GTT TTG ATC ATG TC | CTT TTA TGA TGG TCA ACA GCC TGT GCT G | 2–4 |
| CYP3A5 | TTG TTG GGA AAT GTT TTG TCC TAT C | ACA GGG AGT TGA CCT TCA TAC GTT | TCA GGG TCT CTG GAA ATT TGA CAC AGA GTG CTA | 2–4 |
| CYP3A7 | AAG GGC TAT TGG ACG TTT GAC A | ATC CCA CTG GCC CGA AAG | TAT TTA TGA CTG TCA ACA GCC TAT GCT GGC TAT CA | 2–5 |
| CYP3A43 | CTC TAT ATT TAT GGG ACC CAT TCA CAT A | TTA CAT TCT CTG TCA AAA TTC CAA AGA C | CCT GGG CCA ACC CCT CTG CC | 1–3 |
| hPXR | GCA GGA GCA ATT CGC CAT T | TCG GTG AGC ATA GCC ATG ATC | CCA GCC TGC TCA TAG GTT CTT GTT CCT GAA | 8–9 |