Kinetic parameters (SE) for naproxen demethylation estimated from the biphasic kinetic equation (equation 3) showing activation by p-tolylsulfone at 50 and 100 μM concentrations
| p-Tolylsulfone | Vmax1 | Km1 | Clint3-a |
|---|---|---|---|
| μM | pmol/min/pmol P450 | μM | μl/min/pmol P450 |
| 0 | 2.8 (0.6) | 45.7 (26.5) | 0.01 (.0003) |
| 50 | 19.2 (4.1) | 130.5 (70.8) | 0.003 (.002) |
| 100 | 21.8 (4.4) | 67.0 (54.0) | 0.003 (.003) |
↵3-a Clint =Vmax2/Km2.