Deacylation of 2-formylaminofluorene and 2-acetylaminofluorene by rat liver microsomes and cytosol
| Addition | Deacylase Activity (nmol/30 min/mg protein) | |||
|---|---|---|---|---|
| Microsomes | Cytosol | |||
| 2-Formylaminofluorene | 2-Acetylaminofluorene | 2-Formylaminofluorene | 2-Acetylaminofluorene | |
| None | 0.31 ± 0.04 | 1.43 ± 0.58 | 4.10 ± 0.38 | 0.38 ± 0.22 |
| BNPP | N.D. | N.D. | 3.36 ± 0.82 | 0.28 ± 0.08 |
| Paraoxone | 0.01 ± 0.003 | N.D. | N.D. | 0.01 ± 0.004 |
| PMSF | 0.03 ± 0.01 | 0.04 ± 0.01 | 3.12 ± 0.62 | 0.31 ± 0.05 |
| DFP | N.D. | N.D. | N.D. | 0.02 ± 0.01 |
| KCN | 0.28 ± 0.04 | 1.49 ± 0.16 | 0.90 ± 0.41 | 0.09 ± 0.04 |
Each value represents the mean ± S.D. of three experiments.
N.D. not detected. After 3 min of preincubation with chemicals (1 × 10−4M) with liver preparations. 2-formylaminofluorene or 2-acetyl-aminofluorene was added and incubated for 30 min. 2-Aminofluorene formed was measured by HPLC as described under Materials and Methods.
BNPP: bis(4-nitrophenyl) phosphate, PMSF, phenylmethylsulfonyl fluoride; KCN, potassium cyanide.