Table 1

Comparison of the characteristics of RLIP76 with Pgp (MDR1) and MRP1

TransportersRLIP76Pgp (MDR1)MRP1
Mol. Wt.76 kDa1-a 1-b 170 kDa1-j 190 kDa1-o
Chromosomal LocationChromosome 181-c Chromosome 71-k Chromosome 161-k
TopologyNo clearly defined TMDs. One NBD each in the N and C-terminal domains which are distinct from Walker A and B motifs.1-d 2 TMDs (containing multiple transmembrane helices) and 2 NBDs with Walker A and B motifs.1-l 2 TMDs similar to Pgp with an extra TMD0 (with 5 helices) connected with L0 loop. 2 NBDs. with Walker A and B motifs.1-p
Expression in human tissuesUbiquitously expressed in human tissues including erythrocytes, liver, lung, muscle, kidney, and cultured cells from human origin1-b 1-c ,e-g Widely expressed in human tissues including liver, kidney, brain, pancreas, colon adrenal gland, small intestine.1-m Widely expressed in human tissues including epithelia, muscle cells, and macrophages.1-q
Localization in human tissuesPlasma membrane, nuclear membrane, and cytoplasm1-h 1-i In normal tissues, the apical surface of epithelia. In malignant cells, plasma membrane1-m Cytoplasmic or unidentified vesicular fraction. In malignant cells, plasma membrane1-q
Transport allocrites (substrates)GSH-conjugates, glucuronides, vinca-alkaloids anthracyclins. GSH not required for the cotransport1-b ,d-g Vinca-alkaloids, anthracyclins, taxanes GSH not required for the cotransport1-n GSH-conjugates, glucuronides, bile salts, GSH cotransport required for the transport of vinca alkaloids, anthracyclins1-r

  • TMD, trans membrane domain; NBD, nucleotide binding domain.

  • ↵1-a  Jullien-Flores et al. 1995;

  • ↵1-b  Awasthi et al. 2000;

  • ↵1-c  http://www.ncbi.nml.gov/genome/sts/accessionNM_006788.2;

  • ↵1-d -gAwasthi et al. 1998a,b, 1999,2001c;

  • ↵1-h  Sharma et al. 2001;

  • ↵1-i  Awasthi et al. 2002;

  • ↵1-j  Gottesman and Pastan, 1993;

  • ↵1-k  Dean et al. 2001;

  • ↵1-l  Higgins, 1992;

  • ↵1-m  Thiebaut et al. 1987;

  • ↵1-n  Ambudkar et al. 1999;

  • ↵1-o  Cole et al. 1992;

  • ↵1-p  Bakos et al. 1996;

  • ↵1-q  Flens et al. 1996;

  • ↵1-r  Leslie et al. 2001.