Aglycone | Inhibitor | ||
---|---|---|---|
Control | Etonitazenyl (100 μM) | Buprenorphine (100 μM) | |
pmol/min/mg protein | |||
Anthraflavic acid (20 μM) | 222 ± 4 | 204 ± 21 | 234 ± 6 |
2-Hydroxyestrone (25 μM) | 197 ± 10 | 133 ± 13 | 178 ± 8 |
Assays were performed at 37°C, at optimal pH and incubation times using membrane preparations from cells expressing UGT1A1. The concentration of UDP-glucuronic acid was 2.0 mM and the aglycone concentrations are indicated. The enzyme rates are expressed as the mean ± S.D. of three experiments.