Table 1

Glucuronidation activity towards S-(−)-nicotine, S-(−)-cotinine, and 4-nitrophenol in microsomes prepared from human lung, liver, gastrointestinal tract (stomach, duodenum, jejunum, ileum, and colon), and kidney

TissueS-(−)-NicotineN1-glucuronideS-(−)-CotinineN1-glucuronide4-Nitrophenol-O-glucuronide
V nmol/min/mg protein
LungN.D.N.D.0.065–0.0621-a
Liver0.094 ± 0.0060.020 ± 0.0013.80 ± 0.36
Gastrointestinal tractN.D.N.D.0.10–1.491-b
KidneyN.D.N.D.3.08 ± 0.61
  • N.D., below the limit of detection (0.0015 nmol/min/mg protein for both N-glucuronides); V, UGT activity.

  • 1-a  Lung pools obtained from Dr. Massey (0.065 ± 0.023) and from Human Biologics Institute (0.062 ± 0.029 nmol/min/mg protein).

  • 1-b  Gastrointestinal tract pools of stomach (1.27 ± 0.29), duodenum (1.24 ± 0.05), jejunum (0.87 ± 0.21), ileum (Institute for the Advancement of Medicine, 0.70 ± 0.14; Human Biologics Institute, 1.49 ± 0.13), and colon (0.10 ± 0.04 nmol/min/mg protein).