Glucuronidation activity towards S-(−)-nicotine and S-(−)-cotinine in liver microsomes prepared from eight different species
| Species | S-(−)-NicotineN1-glucuronide | S-(−)-CotinineN1-glucuronide | |
|---|---|---|---|
| V pmol/min/mg protein | |||
| Human | 94.6 ± 6.4 | 19.9 ± 1.1 | |
| Monkey | |||
| Rhesus | 9.97 ± 3.81 | N.D. | |
| Cynomolgus | 6.84 ± 2.04 | tr | |
| Minipig | Yucatan | 2.50 ± 0.42 | N.D. |
| Dog | Beagle | N.D. | N.D. |
| Rabbit | New Zealand White | tr | tr |
| Mouse | CD1 | N.D. | N.D. |
| Guinea pig | Dunken-Hartley | 3.91 ± 0.13 | tr |
| Rat | |||
| Fischer 344 | N.D. | N.D. | |
| Wistar | N.D. | N.D. | |
| Sprague-Dawley2-150 | N.D. | N.D. | |
N.D., below the limit of detection (1.5 pmol/min/mg protein for each N-glucuronide); tr, below the limit of quantification (2.0 pmol/min/mg protein for each N-glucuronide); V, UGT activity.
↵2-150 Untreated or chronically treated with phenobarbital, 3-methylcholanthrene, dexamethasone, or β-naphthoflavone.