RFLP conditions used in genotyping
| CYP3AP1 | CYP3A5*2 | CYP3A5*3 | CYP3A5*4 | CYP3A5*5 | CYP3A5*6 | |
|---|---|---|---|---|---|---|
| Restriction enzyme | FauI | Tsp509I | PstI | BsmAI | Hsp92II | DraI |
| RFLP condition | 55°C 2 h | 65°C 2 h | 37°C 2 h | 55°C 2 h | 37°C 2 h | 37°C 2 h |
| Electrophoresis gel | 2.5% Agarose gel | 4% Nu-Sieve gel | 4% Nu-Sieve gel | 2.5% Agarose gel | 2.5% Agarose gel | 4% Nu-Sieve gel |
| Cutting type | Mutation | Mutation | Mutation | Mutation | Mutation | Wild type |
| 188 bp + 147 bp | 239 bp + 124 bp | 143 bp + 23 bp | 168 bp + 150 bp | 167 bp + 85 bp | 116 bp + 25 bp |