Table 1

Training set substrates

Substrates	K_m	Metabolic Pathway	References
	μm
Amitriptyline	144	N-Demethylation	Ekins et al., 1999c
β-Arteether	28	O-Deethylation	Grace et al., 1998
Benzyloxyresorufin	1.3	O-Debenzylation	Ekins et al., 1998
4-Chloromethyl-7-ethoxycoumarin	34	O-Deethylation	Ekins et al., 1998
3-Cyano-7-ethoxycoumarin	71	O-Deethylation	Ekins et al., 1998
Dextromethorphan	350	N-Demethylation	Ekins et al., 1999c
Diazepam	113	N-Demethylation	Ono et al., 1996
1,2-Dibromoethane	9700	2-Bromoacetaldehyde formation	Wormhoudt et al., 1996
7-Ethoxycoumarin	115	O-Deethylation	Yamazaki et al., 1996
7-Ethoxy-4-trifluoromethylcoumarin	1.7	O-Deethylation	Ekins et al., 1997
Imipramine	383	N-Demethylation	Koyama et al., 1997
Midazolam	46	1′-Hydroxylation	Ekins et al., 1998
Propofol	10	4-Hydroxylation	Court et al., 2001
RP73401	23	Ring hydroxylation	Stevens et al., 1997
S-Mephenytoin	564	N-Demethylation	Heyn et al., 1996
Testosterone	51	16β-Hydroxylation	Ekins et al., 1998

The K_m values were generated using CYP2B6 expressed in β-lymphoblastoid cells.