Individuals | Monkey | Human (Homozygote) | Human H6 (Heterozygote) | Rabbit |
---|---|---|---|---|
n = 3 | n = 2 | n = 1 | n = 3 | |
Unheated | 0.96–2.063-a | N.A.3-b | 0.32 | 10.70–11.64 |
(100) | (100) | (100) | ||
Storage | 0.41–1.04 | 0.20 | 8.81–9.63 | |
(48) | (62) | (85) | ||
Tricine | 0.11–0.20 | 0.08 | 9.04–10.17 | |
(11) | (25) | (88) |
Microsomes were unheated or heated (3.0 μg · μl−1 = high concentration) in storage or tricine buffer at 45°C for 5 min before performing assays under standard conditions (see Materials and Methods) of 0.3 mg of microsomes · ml−1(monkey), 1.0 mg of microsomes · ml−1 (human), or 0.03 mg of microsomes · ml−1 (rabbit).
N.A., no activity detected.
↵3-a The range of individual mean (two assays per individual) activity (nmol · min−1 · mg−1 protein), and percent (in parentheses) retained activity, relative to unheated.
↵3-b Microsomes from twohFMO2*2A/hFMO2*2A individuals were assayed without heating; since activity was not detected, no further assays were performed.