Table 1

Nonenzymatic dechlorination of p,p′-DDT by rat liver microsomes

Additionp,p′-DDD Formed
MicrosomesBoiled Microsomes1-a
nmol/30 min/mg protein
NADH2.2  ± 0.4N.D.
NADPH4.7  ± 0.4N.D.
FMNN.D.N.D.
NADH, FMN6.5  ± 1.112.0  ± 2.1
NADPH, FMN6.3  ± 0.813.1  ± 2.2
NADPH, FMN, CO1-b 0.4  ± 0.3N.D.

Each value represents the mean ± S.D. of four rats. Incubation was performed at 37°C for 30 min with microsomal preparations in the presence of cofactor under anaerobic conditions.p,p′-DDD formed was determined by HPLC. Other details are described under Experimental Procedures.

  • N.D., not detected.

  • ↵1-a  Microsomes boiled for 5 min were used.

  • ↵1-b  Incubation was performed in an atmosphere of carbon monoxide.