Substrate | FMO activity |
---|---|
% of control | |
Methimazole (1 mM) | 100 |
+ 1 mM n-octylamine | 176 |
+ 2.5 mM n-octylamine | 190 |
+ 5 mMn-octylamine | 204 |
Each value represents the mean of three determinations. The rate of methimazole S-oxidation was monitored for 2 min at 37°C; then the substrates were added to the sample and the reference cuvettes, and the reaction rate was recorded for 3 min.