Ki values for the inhibition of (S)-mephenytoin 4′-hydroxylation by (+)-N-3-benzyl-nirvanol and (−)-N-3-benzyl-phenobarbital in human liver microsomes
| Enzyme Source | Ki | |
|---|---|---|
| (+)-N-3-Benzyl-Nirvanol | (−)-N-3-Benzyl-Phenobarbital | |
| μM | ||
| HL134 | 0.23 | 0.071 |
| HL143 | 0.28 | 0.094 |
| HL164 | 0.21 | 0.089 |
| Mean ± S.D. | 0.24 ± 0.04 | 0.085 ± 0.012 |
The activities of (S)-mephenytoin 4′-hydroxylation in three different human liver microsomal preparations were analyzed as described under Materials and Methods. Kivalues were determined by nonlinear regression with SYSTAT Statistics using the competitive inhibition equation.