Ring methyl oxidation and N-deethylation activities toward DEET in individual human liver microsomes
| BALC (Ring Methyl Oxidation) | ET (N-Deethylation) | |
|---|---|---|
| nmol/mg protein/min | ||
| HG0424-150 | 3.30 ± 0.17a | 1.59 ± 0.03a |
| HG0434-150 | 0.54 ± 0.03b | 1.14 ± 0.05b |
| HG0954-150 | 0.52 ± 0.01b | 0.63 ± 0.03c |
BALC and ET activities are based on 5- and 20-min incubations, respectively. Means in the same column followed by the same letter are not significantly different (P < 0.01). Values are the mean ± S.E.M. (n = 3).
↵4-150 Individual human liver microsomes (protein concentration 20 mg/ml). CYP2B6, 2D6, 2E1, 1A2, 3A4, 2C19, and 2A6 activities (picomoles of product per milligram of protein per minute), represented by (S)-mephenytoin N-demethylase, bufuralol 1′-hydroxylase (amount of activity inhibited by 1 μM quinidine), chlorzoxazone 6-hydroxylase, phenacetinO-deethylase, testosterone 6β-hydroxylase, (S)-mephenytoin 4′-hydroxylase, and coumarin 7-hydroxylase catalytic activities, respectively, are 270, 68, 1700, 640, 13,000, 8, and 2300 for HG042 (48-year-old female); 34, 20, 1100, 630, 5600, 700, and 850 for HG043 (23-year-old female); and 12, 160, 1200, 280, 760, 30, and 200 for HG095 (47-year-old female) (data were provided by GENTEST).