Substrates reported to display biphasic kinetics in various in vitro systems
| Substrate | Pathway | Enzyme Source | Reference |
|---|---|---|---|
| Aminopyrine | N-Demethylation | Yeast-expressed CYP1A1 | Inouye et al., 2000 |
| 7-Ethoxycoumarin | O-Deethylation | Yeast-expressed CYP1A1 | Ibid. |
| Levo-α-acetylmethadol | Demethylation | HLM and cDNA-expressed CYP3A4 | Oda and Kharasch, 2001 |
| 1-Methoxy-4-nitrobenzene | O-Demethylation | E. coli-purified CYP1A24-a | Miller and Guengerich, 2001 |
| Naphthalene | Hydroxylation | Baculovirus-expressed CYP3A4 | Korzekwa et al., 1998 |
| Naproxen | O-Demethylation | Vaccinia-expressed CYP2C9 | Korzekwa et al., 1998 |
| Naproxen | O-Demethylation | Baculovirus-expressed CYP2C9 | Hutzler et al., 2001b |
| Nor-levo-α-acetylmethadol | Demethylation | HLM and cDNA-expressed CYP3A4 | Ibid. |
HLM, human liver microsomes.
↵4-a Rabbit enzyme.