Compound | Perfusate Sample | Aglycone2-a | Glucuronide2-b | Sulfate2-b |
---|---|---|---|---|
min | % | |||
Genistein | 0–10 | 50 ± 7 | 10 ± 4 | 2 ± 1 |
10–20 | 60 ± 10 | 12 ± 3 | 1 ± 1 | |
20–30 | 52 ± 14 | 14 ± 5 | 2 ± 2 | |
30–40 | 49 ± 11 | 16 ± 3 | 2 ± 1 | |
Apigenin | 0–10 | 36 ± 6 | 12 ± 3 | N.D. |
10–20 | 42 ± 8 | 11 ± 2 | N.D. | |
20–30 | 41 ± 3 | 11 ± 2 | N.D. | |
30–40 | 40 ± 4 | 11 ± 2 | N.D. |
The recovery was expressed as percentage of original compound in the perfusate (flow rate, 0.382 ml/min). Glucuronide was quantified by using sulfatase-free glucuronidase, and sulfate was quantified by using glucuronidase-free sulfatase.