Table 2

Recovery of original and metabolites of perfused compounds (i.e., genistein and apigenin) as a function of time

CompoundPerfusate SampleAglycone2-aGlucuronide2-bSulfate2-b
min %
Genistein0–1050  ± 710  ± 42  ± 1
10–2060  ± 1012  ± 31  ± 1
20–3052  ± 1414  ± 52  ± 2
30–4049  ± 1116  ± 32  ± 1
Apigenin0–1036  ± 612  ± 3N.D.
10–2042  ± 811  ± 2N.D.
20–3041  ± 311  ± 2N.D.
30–4040  ± 411  ± 2N.D.

The recovery was expressed as percentage of original compound in the perfusate (flow rate, 0.382 ml/min). Glucuronide was quantified by using sulfatase-free glucuronidase, and sulfate was quantified by using glucuronidase-free sulfatase.

  • N.D., not detected.

  • 2-a  Peak time related to the blank perfusate.

  • 2-b  Peaks converted to parent compound then compared with the blank perfusate.