Table 1

Kinetic parameter estimates for 4′-hydroxylation of (S)-flurbiprofen by the CYP2C9 variants

	K_m	V_max	CL_int^1-b	R²
	μM	pmol/min/pmol P450	μl/min/pmol P450
CYP2C9^*1^1-a	19.4 ± 2.1	9.6 ± 0.3	0.49	0.992
CYP2C9^*3^1-a	71.7 ± 31.9	2.5 ± 0.4	0.03	0.944
CYP2C9^*5^1-a	119.7 ± 21.7	1.6 ± 0.1	0.01	0.989
CYP2C9 F114L^1-a	360 ± 111	13.1 ± 2.5	0.04	0.991

Values are the parameter estimates ± S.E. of the estimate.

↵1-a Fitted to the standard Michaelis-Menten equation (model 1).
↵1-b CL_int calculated as the ratio of V_max/K_m, a measure of the efficiency of the enzyme.