Km | Vmax | CLint | R2 | |
---|---|---|---|---|
μM | pmol/min/pmol P450 | μl/min/pmol P450 | ||
CYP2C9*12-a | 89.1 ± 66.0 | 1.39 ± 0.44 | 0.002 ± 0.0002 | 0.990 |
CYP2C9*32-b | —2-c | —2-c | 0.0003 ± 0.00001 | 0.990 |
CYP2C9*52-b | —2-c | —2-c | 0.0005 ± 0.00001 | 0.998 |
CYP2C9 F114L2-b | —2-c | —2-c | 0.002 ± 0.0001 | 0.997 |
Values are the parameter estimates ± S.E. of the estimate.
↵2-a Fitted to the two-binding site model (model 3) so that Km andVmax represent kinetics at binding site 1 and CLint represents kinetics at binding site 2.
↵2-b Fitted to linear function (model 2), where CLint is the turnover rate for that particular isoform.
↵2-c Not applicable since linear kinetics observed. CLint calculated as slope of the line.