UGT | Km | Vmax | Vmax/Km |
---|---|---|---|
μM | pmol · min−1 · mg−1 | μl · min−1 · g−1 | |
1A7 | 129 ± 4.8 | 277 ± 17 | 2159 |
1A8 | 234 ± 24 | 471 ± 100 | 2015 |
1A10 | 49 ± 3.5 | 67 ± 2.0 | 1377 |
Glucuronidation assays were performed using UGT1A7 and UGT1A10-overexpressing baculosomes and UGT1A8-overexpressing HK293 cell homogenates, as described under Materials and Methods. No detectable glucuornidation activity was observed against 7-OH-BaP for homogenates from cells overexpressing UGT1A6 or UGT2B4, or for UGT2B17-overexpressing microsomes. Kinetic parameters were calculated based on three individual experiments for each UGT examined.