Table 1

Kinetic parameters of imipramine N-glucuronidation in human liver microsomes

Human Liver Microsomes	High-Affinity Component		Low-Affinity Component
Human Liver Microsomes	K_M	V_max	V_max/K_M	K_M	V_max	V_max/K_M
	μM	nmol/min/mg protein	μl/min/mg protein	mM	nmol/min/mg protein	μl/min/mg protein
H003	78.9	0.33	4.13	0.73	1.11	1.53
H030	75.6	0.26	4.54	0.42	0.58	0.38
H042	103.2	0.26	2.57	0.58	0.76	1.32
H112	149.2	0.32	2.12	1.09	1.15	1.06
mean ± S.D.	97.2 ± 39.4	0.29 ± 0.03	3.00 ± 0.85	0.70 ± 0.29	0.90 ± 0.28	1.32 ± 0.19

Imipramine (10 μM–2 mM) was incubated for 60 min with 0.25 mg/ml human liver microsomes.