Table 3

Apparent enzyme kinetic parameters for oxidation, glucuronidation and sulfation of galangin by the S9 fractions of human livers

KmVmaxVmax/Km
μM pmol/min/mg μl/min/mg
Oxidation10.5  ± 0.982.9  ± 3.58.0  ± 0.4
Glucuronidation
 Peak 12.8  ± 1.6440  ± 96230  ± 67
 Peak 236.4  ± 11.93961  ± 685133  ± 37
Sulfation0.23  ± 0.0312.1  ± 1.055.7  ± 5.6

Data are means ± S.E. of three determinations.