Table 5

Kinetic parameters describing the hydroxylation of PHT in a pool of human liver microsomes (HHM-0259) in the presence and absence of BSA

BSA	Uncorrected		Corrected^5-c
BSA	K_m	V_max^5-a	V_max/K_m^5-b	K_mu	V_max^5-a	V_max/K_mu^5-b
%	μM			μM
0	16.2 ± 0.7	9.4 ± 0.2	0.58
0.25	4.8 ± 0.3^5-d	10.2 ± 0.7	2.12	3.8 ± 0.2^5-e	10.2 ± 0.7	2.68
2.0	9.3 ± 0.4^5-d	11.3 ± 2.1	1.22	2.7 ± 0.1^5-e	11.3 ± 2.1	4.18
4.0	16.2 ± 0.4	10.4 ± 1.4	0.64	3.2 ± 0.1^5-e	10.4 ± 1.4	3.25

Values are mean ± S.D. from three determinations.

↵5-a The unit ofV_max is pmol/min/mg protein.
↵5-b The unit ofV_max/K_m is μl/min/mg protein.
↵5-c Corrected forf_u.
↵5-d p < 0.05 compared with the control value (BSA = 0%).
↵5-e p < 0.05 compared with the corresponding value of each BSA concentration.