Substrate | Control AdjustedV_{max} | K_{m} | V_{max}/K_{m} | Correlation Coefficient (R) |
---|---|---|---|---|
nmol/min/mg | μM | ml/min/mg | ||
4-Methylphenol | 4.61 ± 0.42 | 1800 ± 230 | 2.56 | 0.998 |
4-Ethylphenol | 2.13 ± 0.10 | 551 ± 54 | 3.87 | 0.997 |
4-n Propylphenol | 0.09 ± 0.01 | 325 ± 58 | 0.28 | 0.986 |
4-iso Propylphenol | 0.28 ± 0.02 | 312 ± 44 | 0.89 | 0.993 |
4-tertButylphenol | 0.20 ± 0.02 | 233 ± 53 | 0.59 | 0.989 |
4-Hydroxyacetophenone | 0.53 ± 0.03 | 833 ± 75 | 0.64 | 0.998 |
4-Chlorophenol | 1.67 ± 0.07 | 246 ± 28 | 6.79 | 0.995 |
4-Bromophenol | 3.00 ± 0.28 | 419 ± 69 | 7.16 | 0.996 |
4-Iodophenol | 2.04 ± 0.11 | 243 ± 28 | 8.40 | 0.997 |
4-Nitrophenol | 1.53 ± 0.2 | 595 ± 140 | 2.57 | 0.987 |
Standard errors in V_{max} were in the range of ±4.0 to ±11.4% of the mean with a mean error of ±7.30%. Standard errors in K_{m} were in the range of ±9.0 to ±22.8% of the mean with a mean error of ±14.8%. The mean of control 1-naphthol activities was 1.51 ± 0.21 nmols/min/mg.