Table 1

Oligonucleotide probes generated for analysis of mdr expression by bDNA signal amplification

GeneGenBank IDTarget1-aFunction1-bProbe Sequence
mdr1a S66618 387–41CE gactggatcctgaatcttttgaagTTTTTctcttggaaagaaagt
S66618 411–436CE tttgcgagttgatcttcttcttattaTTTTTctcttggaaagaaagt
S66618 666–683CE tgctgttctgccgctggaTTTTTctcttggaaagaaagt
S66618 713–736CE aaacgtaatgaaagagatgatcccTTTTTctcttggaaagaaagt
S66618 193–215LE cgtcagcattgcgaactgtagacTTTTTaggcataggacccgtgtct
S66618 362–385LE catgtccacattatcaattccatcTTTTTaggcataggacccgtgtct
S66618 438–455LE catgtggcccacggatgcTTTTTaggcataggacccgtgtct
S66618 550–573LE aatacaccaaccacaaaataaggcTTTTTaggcataggacccgtgtct
S66618 574–595LE gcctccatttattatggcacaaTTTTTaggcataggacccgtgtct
S66618 642–664LE ttcaggggtgtcattttttgtaaTTTTTaggcataggacccgtgtct
S66618 217–237BL ccaccatcaaaaccagcaatg
S66618 239–261BL tgatttccttgctccacaatgac
S66618 262–285BL cctttctctctcatgagctcatca
S66618 286–312BL tgagtcatgacaagtttgaagtaaatt
S66618 313–336BL aattcaatttcatttcctgctgtc
S66618 337–361BL tttagattcacaagcttcatttcct
S66618 456–476BL tgctaagttccccgtcttgat
S66618 481–500BL cgtcgtcatccagagcctct
S66618 503–521BL gccaaaaggaagctggagg
S66618 525–549BL cattcagttgaattcaacttcagga
S66618 597–617BL ttatggagaatgctggttgca
S66618 618–641BL aaacccctacaacctttgaaaata
S66618 686–710BL ggatcaggaacaataaagaaaacaa
mdr1b M81855 2624–2647CE aggataagataattcctgtgccaaTTTTTctcttggaaagaaagt
M81855 2753–2777CE atctctagctctttcttgtccttcaTTTTTctcttggaaagaaagt
M81855 2778–2799CE ttctgtagcgatcttcccagagTTTTTctcttggaaagaaagt
M81855 2919–2938CE gggtgaaggcgaaggtgatcTTTTTctcttggaaagaaagt
M81855 3093–3112CE agactttggccttcgcgtagTTTTTctcttggaaagaaagt
M81855 3394–3416CE ttgagttgctttatttctttgccTTTTTctcttggaaagaaagt
M81855 2579–2597LE gcaagcctggagcccatagTTTTTaggcataggacccgtgtct
M81855 2648–2671LE gctgccagccatagactaagactaTTTTTaggcataggacccgtgtct
M81855 2729–2751LE ggcttgaccagacaacagtttcaTTTTTaggcataggacccgtgtct
M81855 2845–2869LE tctgggcatacatagtttcaaacttTTTTTaggcataggacccgtgtct
M81855 2871–2894LE gcatttctgtatggtatctgcaagTTTTTaggcataggacccgtgtct
M81855 2983–3002LE tctcgtgccaccaagtaggcTTTTTaggcataggacccgtgtct
M81855 3003–3027LE cataacattttcaaacgtcatgagtTTTTTaggcataggacccgtgtct
M81855 3113–3131LE ccaatgatgtgggatgccgTTTTTaggcataggacccgtgtct
M81855 3132–3152LE tcggggattttctcaatgatcTTTTTaggcataggacccgtgtct
M81855 3201–3226LE acttgactccattaaatttcacatttTTTTTaggcataggacccgtgtct
M81855 3294–3310LE ccaccaggcggagcgtcTTTTTaggcataggacccgtgtct
M81855 2598–2623BL ggtttgctacattctgggtaactaca
M81855 2673–2704BL tgagtggtataattactacaagtaaaagtgta
M81855 2705–2727BL ttcaataattccacccaagacaa
M81855 2800–2820BL agtgcggaagttttcaattgc
M81855 2822–2842BL gctcccgagtcaaagagacaa
M81855 2896–2918BL ccaaagacgtgtgctttcttcaa
M81855 2939–2962BL cataggaaaaataaatcatggcct
M81855 2963–2981BL ccgaaccggaaacaagcag
M81855 3029–3052BL caaagacaacagcagaaaatacca
M81855 3054–3070BL tccctgctgccatggca
M81855 3071–3092BL tcaggagcgaatgaactggtat
M81855 3155–3175BL cctccgtgctgtagctgtcaa
M81855 3177–3200BL ccttctaaccaattaggcttcaag
M81855 3227–3246BL gggtcgggtgggatagttga
M81855 3248–3267BL tccctgaagcactgggatgt
M81855 3270–3290BL cccttcttcacctcgaagctc
M81855 3312–3328BL tcccgcagccactgctg
M81855 3329–3347BL agctggaccaccgtgctct
M81855 3349–3368BL gggttgtagaagcgctcgag
M81855 3370–3393BL atctagaaacactgttccagccat

  • ↵1-a  Target refers to the sequence of the mRNA transcript as enumerated in the GenBank file.

  • ↵1-b  Function refers to the utility of the oligonucleotide probe in the bDNA assay (CE, capture extender; LE, label extender; BL, blocker probe).