Primers used for PCR
| Mutant | Orientation | Primer Sequence |
|---|---|---|
| F108L | Forward | 5′-CACAAACCGGAGGCCT-3′ |
| Reverse | 5′-CTGGACCCAAAGGCCTCC-3′ | |
| M371I | Forward | 5′-GATTATTCCCAATTGCT-3′ |
| Reverse | 5′-CTCAAGTCTAATAGCAATTGGG-3′ | |
| S478D | Forward | 5′-CCCCTGAAATTAGATCTAGGAGGAC-3′ |
| Reverse | 5′-GTCCTCCTAGATCTAATTTCAGGGG-3′ | |
| G480Q | Forward | 5′-CCCCTGAAATTAAGCTTACAAGGCCTTCTTCAACC-3′ |
| Reverse | 5′-GGTTGAAGAAGGCCTTGTAAGCTTAATTTCAGGGG-3′ | |
| R212K/D214G | Forward | 5′-CCAAGAAGCTGCTAAAGTTTGGCTTTTTGGATCC-3′ |
| Reverse | 5′-GGATCCAAAAAGCCAAACTTTAGCAGCTTCTTGG-3′ | |
| F219L//T224I/V225L/I230T | Forward | 5′-CCATTGTTTCTATCGATAATCCTCTTTCCATTCCTCACCCC-3′ |
| Reverse | 5′-GGGGTGAGGAATGGAAAGAGGATTATCGATAGAAACAATGG-3′ |
The mutated nucleotides are underlined.