Table 3

Effects of ovariectomy and estrogen treatment on CYP4F expression

SamplePercentage of Female Control (Normalized to r-Cyclophilin)
CYP4F1CYP4F4CYP4F5CYP4F6
Mean ± S.E.
Liver female C100  ± 7.1100  ± 12.9100  ± 7.6100  ± 11.4
Liver female O34  ± 8.23-150 55  ± 6.13-150 94  ± 3.458  ± 7.13-150
Liver female O + E75  ± 103-159 98  ± 4.53-159 101  ± 8.595  ± 1.53-159
Kidney female C100  ± 7.5100  ± 13.6100  ± 11.6100  ± 7.9
Kidney female O20  ± 3.73-160 5  ± 2.23-160 87  ± 7.422  ± 7.83-160
Kidney female O + E63  ± 11.33-159 24  ± 2.13-159 97  ± 1.660  ± 5.93-159

Rats were ovariectomized and were treated with either estrogen or sesame oil as described under Materials and Methods. Assays for each animal were done in triplicate, and data shown represent the average of three different animals each. Data were analyzed using one-way analysis of variance with statistical probabilities.

  • C, control; O, ovariectomized; O + E, ovariectomized plus estrogen-treated.

  • ↵3-160  P < 0.01 and

  • ↵3-150  P < 0.05 when ovariectomized data are compared with control females.

  • ↵3-159  P < 0.05 when ovariectomized female data are compared with ovariectomized + estrogen-treated females.