Formation of carbamoyl glucuronide (M3) in rat, dog, monkey, and human liver microsomal incubations performed under a CO2-enriched atmosphere and in the presence of UDPGA and alamethicin Values obtained from liver microsomes are as follows: rat, pool from 48 male rats; dog and monkey, average from two and four individual animals, respectively; human, average of a commercial pool (n = 10) and four individual preparations.
M3 Formation (% of total radioactivity) | |||||
|---|---|---|---|---|---|
| 50 mM NaHCO3 | 100 mM KH2PO4 (pH 7.4) | ||||
| pH 7.5 | pH 8.5 | ||||
| Rat | 26 | 27 | 9 | ||
| Dog | 28 | 28 | 8 | ||
| Monkey | 2 | <1 | |||
| Human | N.D. | N.D. |
| ||
N.D., not detected