TABLE 2

Irreversibility of the inactivation of human liver microsomal POD activity by zileuton racemate

Data represent mean and S.D. of n = 3 determinations and are expressed as the percentage of control (solvent and NADPH only). Human liver microsomes were incubated in the presence and absence of zileuton (200 μM) for 20 min (see Materials and Methods). Some incubations contained GSH, catalase, or SOD. Dialysis was carried out at 4°C for 4 h, which was sufficient time to completely reverse the inhibitory effects of fluvoxamine (not a time-dependent inhibitor of CYP1A2).