Human P450 proteins that have been targeted with antibodies against their C termini and the specificity of such antibodies The reactivity of these antibodies has been described previously (Edwards et al., 1998).
Human P450 | C Terminus | Antibody Reactivity |
|---|---|---|
| CYP1A1 | -Met-Gln-Leu-Arg-Ser | specific to CYP1A1 |
| CYP1A2 | -Arg-Phe-Ser-Ile-Asn | specific to CYP1A2 |
| CYP1B1 | -Lys-Glu-Thr-Cys-Gln | specific to CYP1B1 |
| CYP2A6 | -Ser-Phe-Leu-Pro-Arg | CYP2A6 and CYP2B6 |
| CYP2B6 | -Arg-Phe-Leu-Pro-Arg | CYP2A6 and CYP2B6 |
| CYP2C8 | -Phe-Ile-Pro-Val | all CYP2C forms |
| CYP2C9 | -Phe-Ile-Pro-Val | all CYP2C forms |
| CYP2C18 | -Phe-Ile-Pro-Val | all CYP2C forms |
| CYP2C19 | -Phe-Ile-Pro-Val | all CYP2C forms |
| CYP2D6 | -Ala-Val-Pro-Arg | specific to CYP2D6 |
| CYP2E1 | -Val-Ile-Pro-Arg-Ser | specific to CYP2E1 |
| CYP3A4 | -Thr-Val-Ser-Gly-Ala | CYP3A4 and CYP3A7 |
| CYP3A5 | -Thr-Leu-Ser-Gly-Glu | specific to CYP3A5 |
| CYP3A7 | -Thr-Val-Ser-Gly-Ala | CYP3A4 and CYP3A7 |
| CYP4A11 | -Asp-Lys-Asp-Gln-Leu | specific to CYP4A11 |