Rat P450 proteins that have been targeted with antibodies against their C termini and the specificity of such antibodies The table summarizes data from several studies (Debri et al., 1995; Edwards, 1998; Schulz-Utermoehl et al., 1999) and includes some previously unpublished data.
Rat P450 | C Terminus | Antibody Reactivity |
|---|---|---|
| CYP1A1 | -Gln-His-Leu-Gln-Ala | specific to CYP1A1 |
| CYP1A2 | -Pro-Arg-Phe-Ser-Lys | specific to CYP1A2 |
| CYP1B1 | -Glu-Glu-Ala-Cys-Gln | specific to CYP1B1 |
| CYP2B1 | -Phe-Ser-Ala-Arg | CYP2B1 and CYP2B2 |
| CYP2B2 | -Phe-Ser-Ala-Arg | CYP2B1 and CYP2B2 |
| CYP2D1 | -Arg-Glu-Gln-Gly-Leu | CYP2D1 and CYP2D5a |
| CYP2D1 | -Gln-Gly-Leu | specific to CYP2D1 |
| CYP2D2 | -Ala-Val-Ala-Arg | specific to CYP2D2 |
| CYP2D3 | -Ala-Phe-Lys-Arg | P450 not detectedb |
| CYP2D4 | -Ala-Ser-Pro-Arg | specific to CYP2D4 |
| CYP2D5 | -Arg-Glu-Gln-Gly-His | CYP2D5 and CYP2D1a |
| CYP2D5 | -Gln-Gly-His | specific to CYP2D5 |
| CYP2E1 | -Val-Ile-Pro-Arg-Ser | specific to CYP2E1 |
| CYP3A1 | -Ile-Ile-Thr-Gly-Ser | specific to CYP3A1 |
| CYP3A2 | -Val-Ile-Asn-Gly-Ala | specific to CYP3A2 |