Kinetic parameters for acyl glucuronidation and glucosidation of compound A by expressed UGT isoforms
| UDP-Sugar | UGT | Km(compound A) | Vmax | Vmax/Km | Ks | Substrate Inhibition Type |
|---|---|---|---|---|---|---|
| μM | pmol(min · mg protein) | μl/(min · mg protein) | μM | |||
| 1A1 | 3.2 ± 0.44-a | 21 ± 0.6 | 6.6 | |||
| 1A3 | 2.3 ± 0.3 | 64 ± 2 | 28 | |||
| UDPGA | 1A9 | 0.23 ± 0.02 | 40 ± 1 | 174 | 170 ± 40 | Uncompetitive |
| 2B7 | 7.6 ± 0.4 | 92 ± 2 | 12 | |||
| 2B15 | 13.0 ± 1.0 | 53 ± 2 | 4.1 | |||
| UDPG | 2B7 | 5.1 ± 0.5 | 104 ± 3 | 20 |
↵4-a Data are expressed as mean ± S.E. from triplicates.