Table 1

Residues in the putative acyl pocket, hydrophobic subsite, and peripheral anionic subsite in M-LK, TGH, HCE-3, HA, and HE

Enzyme	Acyl Pocket^1-150	Hydrophobic Subsite		Peripheral Anionic		Reference
M-LK	LSLQGDTKNSDQFVTS	K	F	W	L	D	A	K	H	T	This study
TGH	LDLLGNPKESYPFLPT	N	Y	W	I	D	V	V	L	T	Dolinsky et al., 2001
HEC-3	LDLLGNPKESYPFLPT	N	Y	W	I	D	V	V	L	T	Mori et al., 1999
HA	LDLLGNPKESYPFLPT	N	Y	W	I	D	V	V	L	T	Yan et al., 1995a
HE	LDLHGDSRQSYPFVPT	N	Y	W	L	D	V	V	L	T	Robbi and Beaufay, 1994

HA, hydrolase A; HE, hydrolase E.
↵1-150 The acyl pocket is equivalent to the region harboring phenylalanines 288 and 290 (the acyl pocket) in acetylcholinesterase (Harel et al., 1992).