Stoichiometry of binding of PCP to P450 2B6
| Sample | % Inactivation | cpm3-a | PCP:P450 2B6 |
|---|---|---|---|
| +PCP, −NADPH, −GSH | 0 | 339 ± 97 | 0.3 ± 0.08:1 |
| +PCP, +NADPH, −GSH | 53.33 ± 1.53 | 5900 ± 402 | 5.5 ± 0.44:1 |
| +PCP, +NADPH, +GSH | 55.00 ± 4.36 | 1230 ± 4 | 1.1 ± 0.06:1 |
Reconstituted P450 2B6 was inactivated in the presence of a trace amount of [3H]PCP. The counts in each sample were measured after extensive dialysis. Each value represents the mean and S.D. of three experiments done in duplicate.
↵3-a Counts per minute for each sample after subtracting the number of counts for the background or control sample and accounting for percent inactivation.