Oxidation of fenthion to fenthion sulfoxide and fenthion oxon by liver microsomes of sea bream and goldfish
| Addition | Oxidation Products Formed | |||
|---|---|---|---|---|
| Sea Bream | Goldfish | |||
| Fenthion Sulfoxide | Fenthion Oxon | Fenthion Sulfoxide | Fenthion Oxon | |
| nmol/30 min/mg protein | ||||
| None | 0.18 ± 0.09 | N.D. | 0.19 ± 0.06 | N.D. |
| NADH | 0.39 ± 0.16 | 0.59 ± 0.17 | 2.74 ± 0.26 | 1.34 ± 0.22 |
| NADPH | 9.29 ± 1.89 | 2.89 ± 0.40 | 10.06 ± 0.32 | 2.80 ± 0.54 |
Each value represents the mean ± S.D. of four fish. Fenthion was incubated at 30°C for 30 min with liver microsomes (2–4 mg protein) in the presence of a cofactor. The oxidation products were determined by HPLC. Other details are described under Materials and Methods.
N.D., not detected.