Primer | Sequencea | Locationb |
---|---|---|
MP-C64T | 5′-ACTCCTGGTTCAGTGCCACCCTAACAC-3′ | 51–77 (27) |
MP-G516T | 5′-CACCTTCCTCTTCCATTCCATTACCGCCAAC-3′ | 501–531 (31) |
MP-C777A | 5′-CCTGGACCCCAGAGCCCCCAAGGAC-3′ | 765–789 (25) |
MP-A785G | 5′-CCAGCGCCCCCAGGGACCTCATCGA-3′ | 773–797 (25) |
MP-C1459T | 5′-CAACATACCAGATCTGCTTCCTGCCCCGC-3′ | 1445–1473 (29) |
All primers were 5′-phosphorylated.
a Bold letters indicate mutations produced by PCR-based site-directed mutagenesis.
b Values in parentheses indicate number of bases (-mer).