Androstenedione | ΔAmax | n | S50/KS1-a | Trough | Peak |
---|---|---|---|---|---|
μM | μM−1cm −1 | μM | nm | ||
0 | 0.048 | 1.62 | 7.8 | 414 | 435 |
(0.001)1-b | (0.16) | (0.5) | |||
25 | 0.040 | –1-c | 4.4 | 413 | 435 |
(0.001) | (0.4) | ||||
50 | 0.036 | –1-c | 2.2 | 413 | 433 |
(0.000) | (0.2) | ||||
100 | 0.033 | – 1-c | 1.3 | 412 | 433 |
(0.000) | (0.1) | ||||
150 | 0.033 | – 1-c | 0.7 | 412 | 433 |
(0.000) | (0.1) | ||||
250 | – 1-d | 397–409 | 433 | ||
400 | – 1-d | 395 | 431 |
↵1-a The S50 andKS value were determined from best fit of the experimental data to the Hill equation, tight-binding equation or hyperbolic equation (Khan and Halpert, 2002).
↵1-b The values in parentheses show the deviation obtained from best fit of the experimental data to the Hill equation, tight-binding equation or hyperbolic equation.
↵1-c These data fit best to the tight binding equation.
↵1-d The binding was too tight in these cases and a good estimate of the binding parameters could not be obtained.