Effect of the addition of androstenedione on binding parameters of wild type P450eryF with 9-AP
| Androstenedione | ΔAmax | n | S50/KS1-a | Trough | Peak |
|---|---|---|---|---|---|
| μM | μM−1cm −1 | μM | nm | ||
| 0 | 0.048 | 1.62 | 7.8 | 414 | 435 |
| (0.001)1-b | (0.16) | (0.5) | |||
| 25 | 0.040 | –1-c | 4.4 | 413 | 435 |
| (0.001) | (0.4) | ||||
| 50 | 0.036 | –1-c | 2.2 | 413 | 433 |
| (0.000) | (0.2) | ||||
| 100 | 0.033 | – 1-c | 1.3 | 412 | 433 |
| (0.000) | (0.1) | ||||
| 150 | 0.033 | – 1-c | 0.7 | 412 | 433 |
| (0.000) | (0.1) | ||||
| 250 | – 1-d | 397–409 | 433 | ||
| 400 | – 1-d | 395 | 431 | ||
↵1-a The S50 andKS value were determined from best fit of the experimental data to the Hill equation, tight-binding equation or hyperbolic equation (Khan and Halpert, 2002).
↵1-b The values in parentheses show the deviation obtained from best fit of the experimental data to the Hill equation, tight-binding equation or hyperbolic equation.
↵1-c These data fit best to the tight binding equation.
↵1-d The binding was too tight in these cases and a good estimate of the binding parameters could not be obtained.