Effect of the addition of ANF on binding parameters of wild type P450eryF with 9-AP
| ANF | ΔAmax | n | S50 | Trough | Peak |
|---|---|---|---|---|---|
| μM | μM−1cm −1 | μM | nm | ||
| 0 | 0.048 | 1.62 | 7.8 | 414 | 435 |
| (0.001) | (0.16) | (0.5) | |||
| 2 | 0.049 | 1.86 | 5.9 | 414 | 434 |
| (0.001) | (0.14) | (0.3) | |||
| 5 | 0.051 | 1.26 | 4.6 | 414 | 435 |
| (0.001) | (0.13) | (0.3) | |||
| 10 | 0.046 | 1.51 | 3.7 | 414 | 435 |
| (0.000) | (0.06) | (0.1) | |||
| 25 | 0.047 | 1.68 | 2.9 | 412 | 433 |
| (0.001) | (0.16) | (0.1) | |||