Effect of the addition of ANF on binding parameters of wild-type P450 3A4 with 9-AP
| ANF | ΔAmax | n | KS | Trough | Peak |
|---|---|---|---|---|---|
| μM | μM−1cm −1 | μM | nm | ||
| 0 | 0.040 | – 3-a | 62.0 | 413 | 434 |
| (0.002) | (7.0) | ||||
| 2 | 0.031 | – 3-a | 17.6 | 411 | 435 |
| (0.003) | (2.3) | ||||
| 5 | 0.051 | – 3-a | 9.4 | 393 | 432 |
| (0.012) | (1.2) | ||||
| 10 | 0.060 | – 3-a | 13.7 | 394 | 432 |
| (0.008) | (2.2) | ||||
| 25 | 0.047 | – 3-a | 21.6 | 394 | 433 |
| (0.006) | (3.5) | ||||
↵3-a These data fit best to the hyperbolic equation.