Table 2

Effect of cryopreservation on viability of rat hepatocytes after 2 h of incubation^2-a

Freshly Isolated Cells	Method 1^2-b	Method 2^2-b
70.3 ± 0.3	57.5 ± 7.0^2-c	70.5 ± 3.2

↵2-a Viability was assessed by trypan blue exclusion after 2 h of incubation in Krebs-Henseleit buffer as described under Materials and Method. Data are expressed in percentage of viable cells ± S.E.M.
↵2-b Isolated rat hepatocytes were cryopreserved following two different protocols: Method 1 and Method 2 as described under Materials and Methods.
↵2-c Viability of cells from Method 1 is significantly different from that of fresh cells (p < 0.05) for n = 4.