Enzyme kinetic parameters of tolperisone hydroxylation in HLM
| OneKm Model | TwoKm Model | ||
|---|---|---|---|
| Low KmComponent | High Km Component | ||
| K m(μM) | 67 ± 2.8 3-a | 7.9 ± 0.95 | 448 ± 58.9 |
| V max (pmol/min/mg protein) | 458 ± 8.2 | 172 ± 9.9 | 436 ± 12.7 |
↵3-a Apparent kinetic values were calculated using nonlinear regression method. Each parameter represents the mean of three determinations ± S.D.