Table 7

In vitro percutaneous absorption of aqueous dilutions of NMP into full thickness skin of male rats

Topical VolumeNeat NMPDilution 1/2Dilution 1/8Dilution 1/16Dilution 1/32
KpmaxTmaxKpmaxTmaxKpmaxTmaxKpmaxTmaxKpmaxTmax
μl/cm2
252.1  ± 0.11.9  ± 0.22.3  ± 0.25.7  ± 0.3
(n  = 6)(n  = 3)
2.3  ± 0.27-a 8.3  ± 1.9
(n  = 3)
503.4  ± 0.12.2  ± 0.1
(n  = 6)
1005.3  ± 0.33.4  ± 0.25.0  ± 0.117.3  ± 1.35.1  ± 1.015.0  ± 3.0
(n  = 4)(n  = 4)(n  = 3)
2006.4  ± 0.33.9  ± 0.35.3  ± 0.122.7  ± 1.3
(n  = 7)(n  = 3)
4.3  ± 0.47-a 25.5  ± 1.5
(n  = 4)
4007.7  ± 0.26.1  ± 0.35.1  ± 0.244.7  ± 1.87.6  ± 1.235.3  ± 4.15.7  ± 1.434.0  ± 8.14.7  ± 0.732  ± 2.0
(n  = 16)(n  = 3)(n  = 3)(n  = 3)(n  = 3)
5.3  ± 0.57-a 46.5  ± 2.1
(n  = 4)
8004.7  ± 0.549.5  ± 1.0
(n  = 4)

Values are expressed as mean ± S.E.M. (two skin samples per rat). A volume of 400 μl of neat or aqueous dilution was deposited on full thickness skin of male rats for 24 h or 52 h.Kpmax (10−3 cm/h) is calculated fromFmax normalized to a skin thickness of 1.3 mm divided by the concentration of NMP.

  • 7-a  Second independent experiment.