Determination of main metabolites of 9-nitro-20(S)-camptothecin in rats by LC/MSn (n = 1-3)
Metabolite Code | Specimen | Identity | Retention Time | [M + H]+ | Typical MS Fragments | ||
|---|---|---|---|---|---|---|---|
| MS2 | MS3 | ||||||
| min | m/z | ||||||
| M1 | Bile, urine | Glucuronide of 9-OH-CPT | 5.4 | 541 | 365 | 321 (100), 336 (7) | |
| M2 | Bile | Loss of glutamic acid from M5 | 5.4 | 541 | 397 (100), 438 (10) | ||
| M3 | Bile, urine | 9AC | 7.0 | 364 | 320 (100), 335 (6) | ||
| M4 | Bile, feces | 9-AA-CPT | 6.2 | 406 | 362 (100), 377 (7) | ||
| M5 | Bile | Glutathione conjugate of 10-OH-CPT | 5.4 | 670 | 541 | 397 (100), 438 (7) | |
| M6 | Urine, feces | Mercapturic acid conjugate of 10-OH-CPT | 7.3 | 526 | 397 (100), 438 (5) | ||
| M7 | Feces | 9-OH-CPT | 8.1 | 365 | 321 (100), 336 (7) | ||
| M0 | Bile, urine, feces | 9NC | 18.4 | 394 | 350 (100), 365 (6) |
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