TABLE 4

Methods to identify pathways involved in the oxidative biotransformation of a compound


In Vitro System

Condition

Tests
HLM ± NADPH P450, FMO vs. other oxidases
HLM, hepatocytes ± 1-Aminobenzotriazole Broad specificity P450 inactivator
HLM 45°C pretreatment Inactivates FMO
S-9 ± Pargyline Broad MAO inactivator
S-9, cytosol
± Menadione, allopurinol
Mo-CO inhibitors