Correlation between rate of formation for clindamycin sulfoxide and standard cytochrome P450 enzyme-specific activities in a panel of human liver microsomal preparations Clindamycin (50 μM) was incubated with human liver microsomes from 12 individual donors (0.3 mg/ml), in triplicate. Activities were normalized to P450 content and correlated with enzyme-specific marker activities [CYP1A2/ethoxyresorufin O-deethylase; CYP2C9/tolbutamide-4-hydroxylase; CYP2C19 (S)-mephenytoin 4′-hydroxylase; CYP2E1/chlorzoxazone 6-hydroxylase; CYP2D6/dextromethorphan O-demethylation; CYP3A4/testosterone 6β-hydroxylase] using standard methods as described under Materials and Methods.
CYP1A2 | CYP2C9 | CYP2C19 | CYP2D6 | CYP2E1 | CYP3A4 | Sulfoxide | |
|---|---|---|---|---|---|---|---|
| CYP1A2 | 1.00 | ||||||
| CYP2C9 | 0.05 | 1.00 | |||||
| CYP2C19 | 0.06 | 0.08 | 1.00 | ||||
| CYP2D6 | 0.00 | 0.02 | 0.00 | 1.00 | |||
| CYP2E1 | 0.21 | 0.00 | 0.19 | 0.21 | 1.00 | ||
| CYP3A4 | 0.00 | 0.27 | 0.16 | 0.07 | 0.00 | 1.00 | |
|
Sulfoxide
|
0.00
|
0.31
|
0.11
|
0.04
|
0.00
|
0.97
|
1.00
|