Conditions for use of conventional and fluorogenic probes in assays
1A2 | 2C9 | 2C19 | 2D6 | 3A4 | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Conventional
|
Fluorogenic
|
Conventional
|
Fluorogenic
|
Conventional
|
Fluorogenic
|
Conventional
|
Fluorogenic
|
Conventional
|
Fluorogenic
| ||||||
| Substrate | Phenacetin | VividBlue | Diclofenac | MFC | S(+)-Mephenytoin | VividBlue | Bufuralol | AMMC | Testosterone | VividRed | |||||
| Buffera,b (nmol) | 19.9 | 21.0 | 20.5 | 20.8 | 20.0 | 21.3 | 20.5 | 20.1 | 20.9 | 20.9 | |||||
| Enzymeb (pmol) | 1.96 | 1.24 | 0.55 | 6.25 | 2.47 | 1.25 | 0.28 | 9.90 | 0.78 | 1.25 | |||||
| Substrateb (nmol) | 10.00 | 1.25 | 2.50 | 7.50 | 5.00 | 1.25 | 1.25 | 0.25 | 10.00 | 1.25E-04 | |||||
|
Ex./Em. (λ)
|
|
405/460
|
|
405/530
|
|
405/460
|
|
390/460
|
|
530/605
| |||||