List of oligonucleotide probes generated for the analysis of mRNA levels of mouse UGTs using QuantiGene signal amplification assay.
UGT Gene | GenBank No.a | Functionb | Sequence | Target |
|---|---|---|---|---|
| Ugt1a1 | L02333 | CE | atactcagccagtggcttccaTTTTTctcttggaaagaaagt | 129-149 |
| CE | ttcgtgccccttctgctgTTTTTctcttggaaagaaagt | 172-189 | ||
| CE | acagggaacttcctcagagtgtaaTTTTTctcttggaaagaaagt | 240-263 | ||
| CE | gtgcagcaggtgggagcaTTTTTctcttggaaagaaagt | 400-417 | ||
| LE | ggaccgaacacacacaacagaaTTTTTaggcataggacccgtgtct | 65-86 | ||
| LE | cccagcgtgggatgcatagTTTTTaggcataggacccgtgtct | 87-105 | ||
| LE | tccatagggaacactaacagcctTTTTTaggcataggacccgtgtct | 106-128 | ||
| LE | gagctgctgaataactccaagcTTTTTaggcataggacccgtgtct | 150-171 | ||
| LE | gcttcaggtgctatgaccacaacTTTTTaggcataggacccgtgtct | 190-212 | ||
| LE | aatgatccttcttttatgtgtattgagTTTTTaggcataggacccgtgtct | 213-239 | ||
| LE | cccttttgactttcatatatattttgaTTTTTaggcataggacccgtgtct | 350-376 | ||
| LE | gaggccataaactcggcattTTTTTaggcataggacccgtgtct | 418-437 | ||
| BL | cacattttccttctggaatggc | 264-285 | ||
| BL | ccaagttccaccaaagtagctgt | 286-308 | ||
| BL | aatcttgattaaaggcagtccgt | 309-331 | ||
| BL | ccacgcgcagcagaaaag | 332-349 | ||
| BL | gccagctaggagcatactggaat | 377-399 | ||
| Ugt1a2 | D87866 | CE | ccctgagattcctcgaagggTTTTTctcttggaaagaaagt | 23-42 |
| CE | ccttcagcccagggcaggTTTTTctcttggaaagaaagt | 63-80 | ||
| CE | tttcttgctgatattcttccttggTTTTTctcttggaaagaaagt | 251-274 | ||
| LE | gcacacatagtcccgtgtccatTTTTTaggcataggacccgtgtct | 1-22 | ||
| LE | gggtaacaccagcacttttgcaTTTTTaggcataggacccgtgtct | 81-102 | ||
| LE | gcacaacatccctcatgctcagTTTTTaggcataggacccgtgtct | 121-142 | ||
| LE | caggaccacagtctggtgacctTTTTTaggcataggacccgtgtct | 159-180 | ||
| LE | ggtcttctcaatgtcgctcagtaTTTTTaggcataggacccgtgtct | 275-297 | ||
| LE | ccttcacaaaatgttgtgttttaaaTTTTTaggcataggacccgtgtct | 298-322 | ||
| BL | gcacacagcaggagcagcag | 43-62 | ||
| BL | ccactggctgccctccat | 103-120 | ||
| BL | cgggcgtggagctccc | 143-158 | ||
| BL | tgtgcacagtcacctctgaagc | 181-202 | ||
| BL | gggtgaagaagtcttctcctttga | 203-226 | ||
| BL | tatatggaaaggcataggttttga | 227-250 | ||
| Ugt1a6 | U16818 | CE | ggaagctgagggtggcagagtTTTTTctcttggaaagaaagt | 523-543 |
| CE | atggctgggtctgtgaacagaTTTTTctcttggaaagaaagt | 566-586 | ||
| CE | acagggcttgggctttgacTTTTTctcttggaaagaaagt | 676-694 | ||
| CE | catgaccggcctgggatattTTTTTctcttggaaagaaagt | 910-929 | ||
| LE | ccttcaggaggctctggcagtTTTTTaggcataggacccgtgtct | 502-522 | ||
| LE | gcatcaaacttgttctccctgaTTTTTaggcataggacccgtgtct | 544-565 | ||
| LE | ccaggatcacaccacagggcTTTTTaggcataggacccgtgtct | 587-606 | ||
| LE | agagggcaggttgagatactcagTTTTTaggcataggacccgtgtct | 607-629 | ||
| LE | caagcatgtgttccagagagcaTTTTTaggcataggacccgtgtct | 654-675 | ||
| LE | gtgtagaatctgggcacataggacTTTTTaggcataggacccgtgtct | 695-718 | ||
| LE | acgtcatgtggtctgagaatttgTTTTTaggcataggacccgtgtct | 719-741 | ||
| LE | actttgaatacagacaataatataggtagttTTTTTaggcataggacccgtgtct | 783-813 | ||
| LE | gaggtctgaggcaatgatttcatTTTTTaggcataggacccgtgtct | 814-836 | ||
| LE | gccacagagaattctggtgtaaggTTTTTaggcataggacccgtgtct | 862-885 | ||
| LE | cgaacacaaaatcgtaccgtaacaTTTTTaggcataggacccgtgtct | 886-909 | ||
| BL | tgggaaacctctgaagaggtagac | 630-653 | ||
| BL | gccagccgttggggaa | 742-757 | ||
| BL | ttccaagatgttaacaatgaagttg | 758-782 | ||
| BL | aaggtagggacacatctctcttgag | 837-861 | ||
| Ugt1a9 | L27122 | CE | ccagcagcctgcctgccTTTTTctcttggaaagaaagt | 42-58 |
| CE | attcagtgatttccccagctgTTTTTctcttggaaagaaagt | 163-183 | ||
| CE | tctgtccaggtcttccagagtgtTTTTTctcttggaaagaaagt | 212-234 | ||
| CE | ccattgagtgtaagataaatacttgaactcTTTTTctcttggaaagaaagt | 235-264 | ||
| LE | tgggccaagccagaggcTTTTTaggcataggacccgtgtct | 25-41 | ||
| LE | tgaatgagtttctccacaaccatcTTTTTaggcataggacccgtgtct | 96-119 | ||
| LE | ccacaacctcatgccctctgTTTTTaggcataggacccgtgtct | 120-139 | ||
| LE | ccaactcacctctgggatgactaTTTTTaggcataggacccgtgtct | 140-162 | ||
| LE | gaacgtatactgtgttctggagttttTTTTTaggcataggacccgtgtct | 265-290 | ||
| LE | tgtcgttaaacaaactcctgcagTTTTTaggcataggacccgtgtct | 339-361 | ||
| LE | ctgcttcaagtactccactaacttctTTTTTaggcataggacccgtgtct | 362-387 | ||
| BL | ttccatccatgggcacca | 59-76 | ||
| BL | tgcatggtgaaccagtggc | 77-95 | ||
| BL | gagaaattgagtaagtcttcactgtaca | 184-211 | ||
| BL | tctggctgaaccagtcaagaaa | 291-312 | ||
| BL | tgtgaaaatgttagttcaaagaaacc | 313-338 | ||
| Ugt2b5 | X06358 | CE | gcagcagcagagcagaaatccTTTTTctcttggaaagaaagt | 26-46 |
| CE | aaggtctcagaacagtgacttcatgTTTTTctcttggaaagaaagt | 163-187 | ||
| CE | aaacttaaggccaggtgatttttTTTTTctcttggaaagaaagt | 212-234 | ||
| LE | aacactttcccacatttcacagaTTTTTaggcataggacccgtgtct | 70-92 | ||
| LE | gaattccatcggccacaccTTTTTaggcataggacccgtgtct | 93-111 | ||
| LE | gtattatttttatgttcatccaatgactTTTTTaggcataggacccgtgtct | 112-139 | ||
| LE | tgggatcaagaacatagtaagctgTTTTTaggcataggacccgtgtct | 188-211 | ||
| LE | tcataagtccacacatccacaaacTTTTTaggcataggacccgtgtct | 288-311 | ||
| BL | tctgaagcagcaacttatctgca | 47-69 | ||
| BL | gcctctctgtaccagttcatcca | 140-162 | ||
| BL | tttactgacagatgtaggaaaagtttc | 235-261 | ||
|
|
|
BL
|
ttaatgaaaaaattttccagattgtc
|
262-287
|
↵ a UGT sequence information is available on the UDP-glucuronosyltransferase website: http://www.unisa.edu.au/pharm_medsci/Gluc_trans/.
↵ b Function of the oligonucleotide probe in the QuantiGene assay: CE, capture extender; LE, label extender; BL, blocker probe.